FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49355658
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG158300.037.11307594
TAAGTCG46550.020.59720894
CAAGTCG126150.019.56004794
GTCGGTT61600.014.2678581
GTTTTTT835750.013.3787611
GTTTATT386150.012.8286931
CGGAAAT102950.012.7829051
GTTTTAT459950.012.7424721
GTCGATT41350.012.73035051
GTTTAAT288700.012.5680651
CGCAAAT61200.012.5179741
CGAATCG23100.012.4112563
GAAGTCG180400.012.19290494
CGAAAAT95850.011.8664591
AATCGGG85150.011.4809165
GTTTTCT683500.011.4422821
GTCGTTT49750.011.2422111
GTTTTTC523950.011.0962881
CGCGTAT10750.010.9302321
CGCGAAT19050.010.8556441
Produced by FastQC (version 0.11.5)