FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41062750
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG80000.036.7187594
CCCGGGT285150.022.7129571
GTCGGTT177750.022.2374111
CGGGTTC313400.020.7255923
CCGGGTT329900.019.6462552
GGTTCAA583500.017.6481575
TAAGTCG69650.017.47738694
GGGTTCA530000.016.8047164
TCGGTTT238550.016.4908812
TTCAAGC500900.016.138957
CAAGCGA310550.014.4685239
TCAAGCG300900.014.4327018
TCCCCTA294150.013.6454182
GAAGTCG161950.013.43686394
CGTCAAT210750.013.2915783
GTTTGCG112400.012.1681495
GTTCAAG928500.011.9714596
CTCGGGT96700.011.9565671
CTCCCCT550900.011.9355591
CCCCTAC328150.011.9021783
Produced by FastQC (version 0.11.5)