FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47714308
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG119700.048.53132694
TAAGTCG42500.045.23058394
GAAGTCG146250.032.77948894
AAAGTCG79250.027.695994
CCCGGGT267700.018.6279411
CGGGTTC275150.017.1669983
CCGGGTT286950.017.0998422
GTCGGTT36000.016.5805551
TCGCGCA42250.015.68520594
TCGCTAT70450.015.14407352
CTCGCTA79500.014.070441
TATACCC196000.013.86020494
CGCTATG77900.013.8164313
GTCGAGT146000.013.53664222-23
CTATCGG103900.012.9826756
CAGTCGA154000.012.89448120-21
TACGTTC120500.012.81286226-27
CTCGGGT111050.012.6546591
TCGAGTT158750.012.55307124-25
GGTTCAA569900.012.4942975
Produced by FastQC (version 0.11.5)