FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40958197
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG168050.062.11663494
TAAGTCG102950.036.0660594
GATATCG45050.027.8557175
ATATCGA47150.026.5153756
GTATCGA39900.025.443616
CGACGGT41100.024.5863761
TATCGAA52500.024.2609527
ATCGATA42650.023.4724528
GACGGTA44200.022.9683272
ACGGTAT43750.022.8822863
CGGTATC43950.022.6712154
TATCGAT45500.022.3120867
GGTATCG46600.021.5836935
GAAGTCG166100.020.88259994
TTAACCC161950.020.1117632
CAAGTCG90900.020.00990194
ATTAACC173700.019.2112851
TCGATAA52600.019.2110259
TCGAATT69600.018.9080479
CGCTCTA68450.018.2987652-53
Produced by FastQC (version 0.11.5)