FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40958197
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG192450.052.99558394
TAAGTCG105100.035.41769894
GTATCGA43350.034.3690876
CGACGGT47050.031.3666291
TATCGAT48300.031.2360237
ATCGATA49150.030.5045788
CGGTATC50300.029.4333974
GGTATCG51750.028.7903395
ACGGTAT52150.028.569513
GACGGTA54350.027.4995382
TCGATAA56800.026.3961269
ATATCGA45500.026.3406566
GATATCG46650.025.4898195
TATCGAA52800.022.6988627
GAAGTCG168600.019.26275394
TATCGGA42300.018.560-61
TTAACCC151800.018.0507262
TCGAATT70350.017.9715719
TCTATCG43250.017.93063458-59
CAAGTCG102800.017.510794
Produced by FastQC (version 0.11.5)