FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6133055
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA33700.09.966158120
GAGTGTA188400.09.550145120
GCCGTAT39050.08.831025118-119
ACGTTAG5550.0094535358.6834745
CCGTATC39500.08.654608120
CGTGTAG424100.08.230465120
CGCCGTA43300.08.172102120
TAGGGAA335700.08.09313120
TGGTCGC67450.08.091501120
ATCTCGG118450.08.050664120
GTGTAGG412850.07.975349120
GTAGGGA360600.07.9500856120
TCGCCGT54400.07.937856120
AGTGTAG176200.07.8968105120
GTCGTGT478500.07.88385120
AGCGTCG585950.07.8301578120
GAGCGTC638700.07.775048120
TAGATCT134400.07.76461120
AAAGAGT261550.07.7046723120
GTAGATC155100.07.579035120
Produced by FastQC (version 0.11.5)