FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54556528
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG402100.059.27306794
TAAGTCG216200.041.76086894
CAAGTCG226950.024.74774494
GAAGTCG389800.022.83684294
AAAGTCG138200.019.75904594
CTAAGTC220400.017.61433894
ATAAGTC160700.016.0566394
TTAAGTC166850.011.28169192-93
ACTAAGT121000.09.32231494
GTCGGAT73050.09.07186994
TCTAAGT160200.08.44943994
GTAAGTC178050.07.91912494
GCTAAGT137950.07.819137692-93
ACAAGTC235000.07.7894
GGAAGTC481700.07.737388694
AGAAGTC467750.07.21453894
GATAAGT105350.07.18272494
CATAAGT121500.07.001646594
TCGGATC84850.06.868592394
CCTAAGT150450.06.62279994
Produced by FastQC (version 0.11.5)