FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49268136
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG113400.027.39594594
GGCGCGT49850.021.4022085
CGCGTGC59300.018.2293437
GAAGTCG103900.017.82290694
TGGCGCG61900.017.2358654
GCGCGTG62950.016.9483726
CAAGTCG77150.014.37718994
GATTCCG86600.012.97113294
AAACGGC173650.011.34062894
AGCCGAT163600.011.06051424-25
GTGGCGC99650.011.0366293
CGGCGAT57550.010.82102690-91
GGCGATC49100.010.8167012
CGCGAAT25900.010.797297582-83
TCCGGCG58500.010.645388-89
CCGATGA173600.010.49107226-27
GCAATCG170400.010.48122168-69
TTCCGAA191900.010.33559294
AGTGCGT275900.010.3233069
TCGAACG122550.010.16319972-73
Produced by FastQC (version 0.11.5)