FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53858873
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA503950.010.5378120
ACACGTC668650.010.517751120
ACGTCTG584050.010.017254120
CACACGT795850.09.771649120
CACGTCT666100.09.594101120
ATCGGAA1693300.09.543255120
GCACACG876900.09.422757120
TCGGAAG1626050.09.188816120
CGGAAGA1551800.09.090985120
GATCGGA1792100.08.829619120
AGATCGG1951250.08.67222120
TAGATCG382500.08.032166120
AAGATCG775200.07.965182120
AGAGCAC1355200.07.797405120
CAGATCG642800.07.78546120
GAGCACA1323850.07.5967765120
AGCACAC1182950.07.5885587120
GAAGAGC1739400.07.082451120
AAGAGCA1724600.06.9449043120
GAGATCG717700.06.8559084120
Produced by FastQC (version 0.11.5)