FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53858873
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG669150.011.474639120
CGTGTAG530700.011.156309120
TCGTGTA560900.010.951334120
AGCGTCG804900.010.888319120
GTCGTGT624350.010.808786120
GCGTCGT719000.010.687417120
GAGCGTC950000.010.121909120
AGAGCGT1033750.09.858943120
ATCGGAA1691300.09.56916120
AAGAGCG1180000.09.552065120
TCGGAAG1634750.09.423151120
CGGAAGA1555000.09.084751120
GATCGGA1802450.08.782713120
AGATCGG1965850.08.693499120
TAGATCG352800.08.314436120
GTGTAGG637300.08.311307120
AAGATCG779900.07.860759120
TGTAGGG679150.07.596005120
CAGATCG662550.07.387951120
GTAGGGA581200.07.3486385120
Produced by FastQC (version 0.11.5)