FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40372431
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG136800.057.7880194
TAAGTCG84800.034.3632194
GTATCGA32450.022.3050866
CGACGGT35400.020.0480211
GAAGTCG144850.019.4035294
ATCGATA37650.018.9747688
TATCGAT37200.018.6989237
CGGTATC37800.018.6507954
CAAGTCG81050.018.4404794
ACGGTAT40450.017.5451163
GGTATCG40350.017.472125
GATATCG38100.017.270345
GACGGTA42650.016.8604932
TCGATAA46450.016.5941879
CGATTGA38450.016.01300490-91
ATATCGA39400.015.8654816
TATCGAA42150.015.1648877
CGCTCTA56950.014.69007952-53
TTAACCC141300.014.668792
ATTAACC150950.014.3537611
Produced by FastQC (version 0.11.5)