FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40372431
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG155850.049.63875294
TAAGTCG85450.034.87185794
GTATCGA35350.028.5855716
CGACGGT36800.027.459241
TATCGAT39900.025.5614037
ATCGATA42200.024.3909958
GGTATCG42650.024.3540465
CGGTATC42100.023.7790974
ACGGTAT43300.023.3371833
TCGATAA46700.022.3426139
GACGGTA48100.021.0083142
ATATCGA39250.017.9617846
GATATCG39950.017.8823535
GAAGTCG150100.017.56628894
TATCGAA42250.016.7976327
CAAGTCG93850.016.62653294
TATCGGA36800.014.81521760-61
TCTATCG36450.014.7640658-59
CTATCGG35900.014.66295260-61
TCGAATT55250.013.6959299
Produced by FastQC (version 0.11.5)