FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19978593
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC832000.09.835751120
ACGTCTG715650.09.699495120
CGTCTGA639950.09.674979120
CACGTCT793250.09.559901120
CACACGT933050.09.497122120
GTCTGAA686050.09.182268120
GCACACG1035750.09.105715120
TCTGAAC630250.08.910035120
AGCACAC1191100.08.613196120
AGAGCAC1398100.08.535186120
CGGAAGA1762950.08.443124120
GAGCACA1329300.08.421803120
TCGGAAG1851100.08.157738120
GATCGGA2069750.08.130765120
ATCGGAA1931850.08.065197120
AAGAGCA1560900.07.994746120
AGATCGG2224850.07.9225955120
CTGAACT625600.07.863819120
TGAACTC631450.07.638947120
GAAGAGC1708700.07.317258120
Produced by FastQC (version 0.11.5)