FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41568604
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG71800.032.79526594
GTCGATT32500.025.3076921
CGATTTG51400.014.630353
GCGACAT43150.014.0509851
GTTTTTT1152650.013.9493341
CGCGAAT11150.013.9103141
GTCGAAT28000.013.7642861
TCGATTT68700.012.8617172
CGAAAAT73950.012.3299531
GTTTGAT266750.012.28078651
CGGAAAT66200.012.0694871
GTTTTAT565900.012.0676791
TTGAACC431750.011.9309792
CGAGATT100350.011.8963631
TTGGCGA49650.011.832837
TAAGTCG37050.011.79757194
CGATCCC80950.011.7862863
GTCGGTT41400.011.4661841
GTCGGAG29950.011.455761
CTTGAAC626450.011.4189481
Produced by FastQC (version 0.11.5)