FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21975277
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN899950.40952839866364366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA299800.011.229485120
GCGTCGT386150.010.894079120
CGTGTAG283850.010.866828120
GAGCGTC510900.010.782894120
AGCGTCG432400.010.644819120
CGTCGTG361200.010.550051120
GTCGTGT336050.010.357446120
AGAGCGT551200.09.493708120
ATCGGAA869950.09.340131120
GATCGGA930450.09.229436120
TCGGAAG844800.08.943354120
CGGAAGA810700.08.875392120
AAGAGCG613750.08.829371118-119
AGATCGG1013250.08.439696120
GTGTAGG330000.08.237831120
GTAGGGA294700.08.002782120
TGTAGGG351550.07.6987114120
CAGATCG376000.07.692859120
TAGGGAA300650.07.445197120
TAGATCG157150.07.389246118-119
Produced by FastQC (version 0.11.5)