FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34247774
Sequences flagged as poor quality0
Sequence length126
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG393300.011.265659120
CACTTAG445050.011.132527120
CTCGTAT117400.010.768469120
TCACTTA529900.010.565542120
CTTAGGC378850.010.328774120
ACGTCTG2139350.010.240071120
CGTCTGA1929900.010.228438120
GTCTGAA1877850.010.005426120
CTGAACT1642950.09.95195120
GTCACTT645000.09.912143120
TCTGAAC1717700.09.85532120
CACGTCT2359350.09.754717120
ACACGTC2512050.09.744103120
TTAGGCA340250.09.713527120
TGAACTC1590150.09.661517120
AACTCCA1335700.09.591034120
AGTCACT727450.09.516942120
CACACGT2778500.09.331839120
TCCAGTC1045200.09.238633120
CCAGTCA947400.09.112101120
Produced by FastQC (version 0.11.5)