FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49617550
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA9648721.9446183860347799No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC8754981.764492603927441No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC7660231.5438549464856688No Hit
CGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACA6589461.32805025641129No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC3936800.7934289379463517No Hit
GGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGG3438860.6930733178079127No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT3144730.6337938894604832No Hit
GTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGC3062270.6171747698143096No Hit
GTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAA2867400.5779003598525119No Hit
GCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGA2860740.5765580928522267No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG2752610.5547654005487977No Hit
GTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCG2734910.5511981143768687No Hit
GGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGG2588250.5216400245477658No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC2430300.4898065301491106No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAG2297310.46300351387765015No Hit
GGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATT2187710.4409145554345187No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC1925540.38807639635572494No Hit
CCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCT1869530.3767880518082815No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT1840830.37100380812837397No Hit
CAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGGTATTTGGA1713130.34526694687665954No Hit
GGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGG1705020.3436324445685045No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT1703330.3432918392786423No Hit
GCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAA1702870.3431991301464905No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT1684150.33942627155109434No Hit
CGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTG1679980.3385858431139788No Hit
GGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGGT1591330.3207191810155882No Hit
GCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGG1485570.2994041422843329No Hit
GGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGT1479050.2980900911068765No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG1461780.2946094678193502No Hit
TGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTG1453970.2930354279886855No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCC1441030.29042747979293615No Hit
CAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACC1401140.28238798570263945No Hit
GCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCC1357270.27354635607763783No Hit
CAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGC1355400.2731694733012815No Hit
CCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGG1327800.26760692537217173No Hit
CACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTT1315530.2651340100428175No Hit
CAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGA1305240.2630601470648994No Hit
GGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCA1285020.2589849760820516No Hit
CAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGC1276090.2571852096687563No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA1250640.2520559761616606No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC1246940.2513102722726132No Hit
CTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTG1200560.2419627732526092No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT1180710.23796217265866612No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC1143000.23036203923813248No Hit
CCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGGTATTTGG1076730.21700587796052No Hit
CGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGT1057340.21309798649872877No Hit
CCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTC1012120.2039842757250207No Hit
GTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACG995390.20061248489697697No Hit
CTCAGACTTTATTCAAAGACCACGGGGGTACGGGTGCAGGAAGGGGAGGA992240.1999776288833286No Hit
CCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAAC980110.19753292937680317No Hit
GCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCT953680.19220618510990567No Hit
GCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAG952290.19192604229753385No Hit
GCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTT948100.19108158302858566No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC946740.19080748646396287No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA904770.1823487858630666No Hit
CTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTT883790.17812044327057663No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC870160.1753734313765996No Hit
GGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGGTATTTGGAGGT840500.16939570776872295No Hit
GGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGGTATTTGGAGGTC823350.16593926947219279No Hit
TTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAG810420.16333333669235986No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT801080.16145093822649445No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA787780.1587704350577568No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT777340.15666634084109354No Hit
TGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACG751340.15142625945859883No Hit
GGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCG751020.1513617661492758No Hit
GCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGGTATTT741890.14952169141765362No Hit
GGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGGTATT734330.1479980369848975No Hit
CAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCG726020.14632322635841552No Hit
CTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCG713880.14387651143597377No Hit
GTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGA708920.14287686514146708No Hit
CTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAA705050.14209689918184193No Hit
GGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCAC698790.1408352488182105No Hit
CTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCT692370.13954135179991758No Hit
CAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGA688910.13884401789286252No Hit
CTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTGGCCATGCTTCTTGC686530.13836434890477262No Hit
GGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGG685050.13806606734915366No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA684980.13805195943773926No Hit
GCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTC684180.13789072616443174No Hit
CCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGAC676700.13638319505900634No Hit
GGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGT665360.13409771340987212No Hit
GTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGC659940.13300535798321358No Hit
GCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGG657710.13255592023386886No Hit
CACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCT653150.13163689057601594No Hit
CAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCA649050.13081057005031485No Hit
GGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAAC648230.13064530594517465No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT638970.12877903080664No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG638370.12865810585165935No Hit
GTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCA634400.12785798573287074No Hit
CTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCA607440.122424424422407No Hit
GCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGC600540.12103378744012955No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC598280.12057830344303579No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC597770.12047551723130222No Hit
CCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCC593560.11962702713052137No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA592340.11938114638872738No Hit
CCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCT590760.119062710673945No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG582630.11742417753395724No Hit
GCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGC580790.11705334100534992No Hit
CTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCT574770.11584006062371076No Hit
CCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCT553600.11157342512881027No Hit
GCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCA546750.11019286522611456No Hit
AGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAG538710.1085724708293739No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT538510.10853216251104698No Hit
TGGCCACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC536200.10806660143437151No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAG535020.10782878235624291No Hit
GACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCT530530.10692386060980438No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT530410.10689967561880827No Hit
GCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCG523970.10560174776868266No Hit
CGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCC520540.10491046010937663No Hit
CCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCC510450.10287690544978541No Hit
GTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGG510170.10282047380412776No Hit
AGCATGGCCACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT509190.10262296304432605No Hit
CACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAA507530.10228840400221292No Hit
CTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGT499130.10059545463248387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG130750.068.62179694
AGAGAAC241200.038.971811
TTAGGCA111450.037.5325249
TCAGACT483300.036.6139032
CAAGTCG120050.034.4523194
TCGGTAG1392100.034.1974794
CTCAGAC517500.033.458551
AGACTTT526850.032.9450534
CAGACTT541900.032.3683323
GAGAACC317750.031.8017312
GAATCCA230300.030.8163264
ACTTTAT579850.029.9256676
GACTTTA582250.029.7215985
AGAATCC232300.029.6000863
CCACTCA289550.029.4774652
CAGAATC230100.029.352022
GAAGTCG111750.028.89395994
TCGGTGG1242650.028.80931594
GCAGAAT250900.027.199681
CACTCAG715300.027.19600194
Produced by FastQC (version 0.11.5)