FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13131288
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN709940.5406476501010411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG222850.010.691166120
GCGTCGT200500.010.655721120
TCGTGTA155700.010.329835120
CGTCGTG188050.010.308046120
CGTGTAG152200.010.133645120
GTCGTGT175600.09.671841120
AGAGCGT280750.09.405461120
ATCGGAA444300.09.130984120
AAGAGCG318550.09.042965120
GTGTAGG178650.08.902051120
GATCGGA472800.08.809052120
GAGCGTC262250.08.718397118-119
TCGGAAG442150.08.686755120
AGATCGG518050.08.595664120
CGGAAGA427700.08.2646265120
AAGATCG148400.07.7240787120
GTAGGGA157250.07.5946827120
CAGATCG217550.07.3378663120
GAAGAGC489900.06.8723087120
GAGATCG238600.06.8665633120
Produced by FastQC (version 0.11.5)