FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46304436
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA582590.12581731910091726No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA475660.10272449922508503No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG475630.10271802036418282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG156650.071.2575894
TAAGTCG48600.036.07201894
CAAGTCG134550.035.31549594
GAAGTCG153500.030.49641894
CGTCGGA168450.024.63698694
GGTTCGA96400.022.6711624
AATAGCG169050.021.39396590-91
TAGCGGC169800.020.579892-93
GTTCGAT111950.019.3961581
TCGATTC118950.018.096687
CAACCCG46950.018.0191691
GGGAATG257100.017.6044332
TTCGATT123300.017.5725866
GGCGGAT293300.017.1142181
AAAGTCG61400.016.53420394
CGGATGA251750.016.5223433
TCCGTTG206250.016.2249726-27
AAACGAA241150.015.9720178-79
CCGTCAT79500.015.8440271
ACAAACG245150.015.83601976-77
Produced by FastQC (version 0.11.5)