FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45993256
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG168300.050.96553894
TAAGTCG41850.028.63799594
CAAGTCG146500.026.14675994
CGTCGGA103050.021.39058794
GAAGTCG178050.018.4515694
GGCGGAT239400.014.9795321
GTTCGAT90300.014.1572545
GTCGGAA114950.013.9016961
GGGGAAT320750.013.5688251
GGTTCGA99950.013.4487254
GTACGAT56500.012.89380559
TCGATTC102850.012.8867287
CGACCGT32850.012.4474891
AATAGCG44750.012.23575490-91
TAGCGGG166100.012.13907294
TTCGATT109500.012.0611876
TCAATCG131700.011.6340178
GGGTTCG130450.011.3491753
CAATCGG129750.011.3017349
ATACGGA94800.011.27900956-57
Produced by FastQC (version 0.11.5)