FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45993256
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG183400.046.8718794
TAAGTCG42250.026.58698594
CAAGTCG157750.025.71220294
GAAGTCG185400.017.23840394
CGTCGGA137000.015.95255694
GGCGGAT225150.014.862981
CGACCGT33250.014.4180461
GTACGAT53750.013.29116259
GTTCGAT89400.013.1431775
CGTATCG65000.012.87076953
GGTTCGA101900.012.637884
GGGGAAT312250.012.2975181
GGCGGTT108250.012.1570441
GCGGGTT74300.012.08211
TAGCGGG152400.011.78083994
GTCGGAA120800.011.6332781
CGAATGT185300.011.4392888
GGGGGAT279350.011.4240211
TCGATTC106800.011.3979417
GTATCGA71900.011.3741314
Produced by FastQC (version 0.11.5)