FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47119850
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG168150.057.8031594
TAAGTCG47500.030.17894694
CAAGTCG148300.029.06203794
CGTCGGA110750.021.38871494
GAAGTCG194100.021.06646294
GGTTCGA89400.017.3489954
GTTCGAT88750.016.8935225
AATAGCG58650.015.06564390-91
TCGATTC100900.014.8126867
TTCGATT104100.014.5379456
GGCGGTT85600.014.3306061
GGGGAAT332850.013.8663051
GGCGGAT248700.013.8335341
ATGCGTA80950.013.26683156-57
GGGTTCG147050.012.6888833
GTCGGAA118000.012.4669491
CGTATCG54000.011.6629633
AAAGTCG90150.011.46977294
TCGATTG126750.011.43944824-25
TCAATCG154400.011.4151558
Produced by FastQC (version 0.11.5)