FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46742610
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG565730.12103089664868949No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA502280.10745655837361243No Hit
GTTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATG468030.10012919689336988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG169600.069.7794894
TAAGTCG52800.037.03030494
CAAGTCG139250.036.31741394
CGACCGT25950.035.1368031
GAAGTCG145750.033.53687794
CGTCGGA182100.028.57166594
GGTTCGA111150.025.8362584
AAAGTCG54900.024.57012794
CAACCCG56900.023.2108961
ACCGTTG40100.022.3865343
GTTCGAT127700.022.0830085
GGCGGAT290950.021.9371031
AATAGCG149900.021.2738590-91
CGGATGA235450.021.1594813
TCGATTC135550.020.9428277
CCGTCAT105500.020.6265411
TAGCGGC147900.020.57640392-93
GACCGTT43800.020.3881282
TTCGATT142850.020.0371026
GGGTTCG158050.019.9240743
Produced by FastQC (version 0.11.5)