FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46742610
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA561890.12020937641265646No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG537670.11502780867392728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG183950.064.2337694
CAAGTCG158100.037.130394
CGACCGT26900.035.6431241
TAAGTCG51300.035.27290794
GAAGTCG145400.029.18913394
CAACCCG66200.027.1208461
CGTCGGA188300.025.85873894
ACCGTTG40400.023.3836653
AATAGCG136900.023.25967890-91
GGTTCGA114050.022.8715484
GACCGTT41250.022.673942
GGCGGAT252450.022.6203211
CCGTCAT123850.022.3899881
TAGCGGC136000.022.22132192-93
GTTCGAT119600.021.2600355
GACGCTA78450.020.4295714
CGTCATC133200.020.359612
CGGATGA245800.019.9052093
CGCGGAT16650.019.759761
TCGATTC131150.019.6027457
Produced by FastQC (version 0.11.5)