FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34543368
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG49600.054.29636894
GCGACAT25450.029.3634591
CGACATC33950.022.8424152
CAAGTCG42600.022.28638394
GAAGTCG51550.021.88166694
TAAGTCG29500.021.34915494
CCCTACG40400.019.4282179
GTTTTTT1183400.014.4725361
GTCGATT24550.013.9755591
CGTCGGA17950.013.87743772-73
CTCGAAT35750.013.8041951
TACGCCG12950.013.7915059
CGCGATC33300.013.6906911
AGACCGG49950.013.07907994
CTCGGAT25950.013.04046251
GCGGAAT15400.012.8181811
CGCGAAT4052.5925856E-612.7654311
GTTTTAT700800.012.6017411
CGGACAT20850.011.9472431
AAAGTCG42100.011.94536894
Produced by FastQC (version 0.11.5)