FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34543368
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG59100.045.40947394
GCGACAT24150.026.0786741
CAAGTCG47100.024.84713294
TAAGTCG30950.019.43780194
CGACATC32350.018.4513132
CCCTACG39850.018.3989969
GAAGTCG51050.016.47992194
GTTTTTT1093450.014.0813021
CGCGATC28650.013.6160551
CGCGAGT6751.8189894E-1213.22962951
CTTGAAC362850.012.6162321
CGAAAAT60400.012.605961
CTCGAAT38250.012.5333331
CGAAAAC45400.012.3193841
GTCGGAT16050.012.2990661
GTTTTAT682350.012.2881221
CGCGGAT6904.9112714E-1112.2608691
GTCGATT22850.011.9299781
AAAGTCG47300.011.82452494
AGACCGG49750.011.80904594
Produced by FastQC (version 0.11.5)