FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24832835
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN878880.3539185115191238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG254550.09.524239120
AGCGTCG366400.09.499363120
CGTCGTG312650.09.385824120
TCGTGTA253950.09.180487118-119
AAGAGCG526150.08.9760895120
GCGTCGT323600.08.91987120
GAGCGTC444400.08.898838120
GTCGTGT284400.08.841103120
ATCGGAA693600.08.790359120
AGAGCGT457300.08.765915120
GATCGGA737050.08.630399120
AGATCGG812700.08.617129120
TCGGAAG705550.08.318271120
CGGAAGA693050.08.026702120
TAGATCG85900.07.894184120
GTGTAGG312100.07.4411345120
CAGATCG389200.06.660913118-119
GTAGGGA289850.06.293935120
AAGATCG249650.06.2016907120
GAGATCG381300.06.153638120
Produced by FastQC (version 0.11.5)