FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46935855
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG160450.057.17918494
GAAGTCG153300.046.0802494
AAAGTCG80650.040.56044894
TAAGTCG38500.036.501394
GAGATAC177200.016.89563
ATACCAT185700.015.1351656
CGAAGTC89950.014.94385994
GATACCA198850.014.3706315
AGCGCGT284700.014.29645259
AGATACC206700.013.98403554
GGCGATC88450.013.9751271
GGGCGAT84150.013.7956041
GGAGATA235300.013.6425852
GGGAGAT352950.012.7570471
ACAAGTC194900.012.75679894
GAGCGCG322650.012.6294768
CCAAGTC401600.012.5575294
TCACGAA152450.012.36274214-15
TCAATCG57150.012.3359583
GGCGGCT284200.011.3944411
Produced by FastQC (version 0.11.5)