FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45685377
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG189650.051.5971594
CAAGTCG170150.028.23038394
TAAGTCG49750.027.77487494
GAAGTCG197250.018.96679394
GTTCGAT96450.018.8584775
GGTTCGA107500.018.1879064
CGTCGGA163350.018.15549394
GGCGGAT229550.017.3217161
TTCGATT111550.016.4742266
AATAGCG61600.016.02272690-91
TCGATTC116400.015.78787
TACCGTA71400.015.600849
GGGTTCG138600.015.4292933
GACGCTA75550.014.7438774
CGTATCG80400.014.5559693
CGACCGT39150.013.6858231
CGAATGT219900.013.4652118
CGGATGA235550.013.4485253
TAGCGGG178300.013.33819394
TCAATCG186950.013.2992778
Produced by FastQC (version 0.11.5)