FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73274081
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAT780200.06.34337331
CTTAAAT390900.06.25223831
CTCAAAT438100.06.08285761
CTGAAAT711800.05.94268041
CTAAAAT495850.05.9052141
CTTAAAC254550.05.72382641
CTAAAAC327200.05.7169931
AAATCCA650900.05.54555234
CTGAGAT706550.05.49458651
AAAATCC530200.05.46944573
CTTGAAT402900.05.45941931
CAAAAAC612400.05.4337041
CTGGAAT709600.05.43122861
AAAAATC714000.05.3977592
CTGAATT551750.05.38359741
AAATCTA386700.05.37212324
GGAAAAT728400.05.3233121
TGAAATC513650.05.2247642
CTCAATT295000.05.20983081
AATCTAG195700.05.18753245
Produced by FastQC (version 0.11.5)