FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48972404
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG135650.042.33984494
TAAGTCG41600.022.14423294
CAAGTCG112500.021.72444394
CGTCGGA85500.017.86549694
CGACCGT31350.016.1913871
GAAGTCG168950.014.52145594
CGTATCG55850.013.6329463
GTCCGTT39500.013.2075941
GTACGAT50650.012.7127349
GGGGAAT325900.012.3160491
GTCCGAT36750.012.2775521
GTCGGAA98300.012.048831
GGTTCGA79750.011.9636354
GTCGTGT106150.011.9547811
GTTCGAT72950.011.5325575
CCGTATC81800.011.4339862
GGCGGAT227950.011.4020611
TATAGGT146100.011.22724157
ACCGTTG44050.011.096483
GGGGGAT330300.011.0705421
Produced by FastQC (version 0.11.5)