FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48972404
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG149500.038.38595294
CAAGTCG121800.022.14942694
TAAGTCG42400.021.2830294
CGACCGT35200.017.7585221
GAAGTCG172550.014.02781994
CGTCGGA83450.013.96764794
CGTATCG52950.013.2256843
ACCGTTG46100.013.0498913
GGTTCGA92100.012.6558094
GTACGAT43250.012.605789
GTCCGAT35900.012.5682451
GTTCGAT84600.012.3333335
GGGGAAT306200.011.72697451
GTCCGTT44300.011.5643351
GGGGGAT296100.010.9206351
TTCGATT100750.010.68287856
GGCGGAT203800.010.6545631
TCGATTC99300.010.5075537
TACCGTA59500.010.5058829
CCCGTAT94050.010.4944171
Produced by FastQC (version 0.11.5)