FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33343539
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG94800.010.693781120
GTCGTGT1352100.09.392149120
CGTGTAG1174400.09.352447120
CGTCGTG1461250.09.191418120
TCGTGTA1234000.08.910462120
AGCGTCG1704050.08.85337120
GTGTAGG1177850.08.800487120
GAGCGTC1894700.08.557729120
GCGTCGT1553400.08.495565118-119
TAGGGAA992500.08.376945120
AAGAGCG2219550.08.2538595120
GTAGGGA1032550.08.028786120
AGAGCGT2020250.07.919009120
TGTAGGG1142100.07.8469234120
GATCGGA3215850.07.565804120
ATCGGAA3017550.07.5540886120
TCGGAAG2908100.07.4732914120
CGGAAGA2780500.07.361038120
AGATCGG3446800.07.293811120
AGTGTAG511550.07.282097120
Produced by FastQC (version 0.11.5)