FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46175784
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA614660.13311306203268794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT103550.022.9086631
GGCGATC102400.022.8441892
GAGATAC337050.022.0917223
ATACCAT338600.021.2261126
GATACCA357050.020.4192735
AGATACC369400.019.825864
GGAGATA416500.019.0981372
GGGAGAT547550.018.0171811
TACCATG455300.016.0026957
ACCATGA475100.015.098018
GGCGCGT240350.014.667534
CGATCTG165350.014.289564
TGCGACA126450.013.91078312-13
CCATGAT540350.013.4228999
TCACGAA381350.013.11731314-15
CGCGTGC272300.013.06756
TGGCGCG270600.012.9235064
CGACATC142850.012.31375814-15
ACGAAGG416650.012.07927116-17
GCGCGTG313050.011.4867615
Produced by FastQC (version 0.11.5)