FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21733237
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1164220.535686423518043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA233150.011.607839120
CGTCGTG277600.011.111019120
GCGTCGT297000.010.789344120
AGCGTCG332600.010.680944120
GTCGTGT257550.010.135331120
GAGCGTC392300.010.049798120
CGTGTAG224750.09.9590845120
AGAGCGT427750.09.679866120
AGATCGG776500.08.956786120
GATCGGA708450.08.893861120
TCGGAAG660200.08.653098120
AAGAGCG487050.08.562912120
CGGAAGA646650.08.388984120
ATCGGAA670700.08.383426120
GTGTAGG271300.07.763679120
CAGATCG292650.07.4843583120
AAGATCG257300.07.439832118-119
TAGATCG120450.07.3733554120
GTAGGGA250650.07.1344213120
GAGATCG347100.07.0018153120
Produced by FastQC (version 0.11.5)