FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74416726
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAT839800.07.0292921
CTGAAAT750950.06.8846131
CTAAAAC352700.06.3164161
GGAAAAT788950.06.24919181
CTAAAAT528450.06.01231
CTTAAAT396550.05.87870361
AAAATCC560600.05.83517653
AAATCCA695500.05.79813054
CTGAGAT713300.05.77863451
CTCAAAT453700.05.7701121
CTGGAAT726600.05.73107621
TGAAATC533850.05.7137772
CGAAAAT92250.05.70623351
CAAAAAC653950.05.6993651
AAATCTA410000.05.674394
AAAAATC765800.05.67093232
CGCGAAT19958.9787136E-85.6541351
CTTAAAC262350.05.57156471
AATCCAG592900.05.52521475
CTGAAAC600850.05.5146871
Produced by FastQC (version 0.11.5)