FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74416726
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAAT757800.07.07046651
CAAAAAT828300.06.8431731
CTAAAAC349200.06.5816151
CTAAAAT500900.06.43681341
CAAAAAC658750.06.2785581
TGAAATC529000.05.988282
CTGAAAC602150.05.93988231
CTGAGAT715650.05.85160351
CTTAAAC262750.05.77773571
AAATCCA691950.05.73278434
CTGGAAT712800.05.71675061
CTTAAAT384650.05.6451321
AAAAATC739050.05.6090932
CGCGAAT21054.6971763E-85.5819481
GAAAATC548300.05.5717672
AAATCTA390150.05.5535054
AATCCAG584250.05.52657275
GGAAAAT764200.05.51674941
TTAAATC350550.05.4970762
CTGAATT564750.05.45108461
Produced by FastQC (version 0.11.5)