FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73242453
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAT818500.07.1490531
CTGAAAT764550.06.54698851
CTAAAAT527100.06.39328431
CTAAAAC355900.06.3256531
GGAAAAT783750.06.22468951
CTTAAAT413450.06.13859031
AAAAATT1239950.06.01548432
CTGAGAT711750.05.92989161
CGAAAAT94050.05.84688951
CTGGAAT727200.05.84268431
CAAAAAC641150.05.84247061
CTCAAAT461700.05.81264931
AAAAATC755750.05.80853462
AATTAGC303850.05.7541555
GAAAATC556650.05.7161592
TGAAATC540950.05.68222572
AAAATCC557050.05.6614313
AAATCCA685850.05.64671564
AATCTAG204950.05.6184445
CTGAATT575050.05.60681721
Produced by FastQC (version 0.11.5)