FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49182651
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT844310.17166825757318369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG320000.085.20219494
CAAGTCG206100.040.2954994
TAAGTCG136800.039.40716694
GAAGTCG277850.039.14270494
AAAGTCG135500.032.98671794
CGTCCCG65700.023.9649933
CCGCGTT20450.021.1442557
ACCGCGT19700.020.9949256
TATACCC408550.019.74103594
GGAGTCT468000.019.5632483
CGCGTTC22300.019.3901358
GACCGCG21600.019.3657425
GTCTTGG497550.019.1192846
TGGAGTC489150.018.8230612
CCCGTCC98500.018.561421
GAGTCTT495550.018.4376964
CTCGCTA52650.018.3000951
AGTCTTG555000.017.3857655
CCCTACG265800.017.3288224-25
CCCGTAT46900.016.9360331
Produced by FastQC (version 0.11.5)