FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49182651
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG349700.077.8450294
CAAGTCG212200.039.46936894
GAAGTCG345750.038.53796494
TAAGTCG110050.036.25897294
GGTCGAC69400.030.6109521
CGACCCG72100.029.2690734
CCGTGCG81600.026.2071088
GTCGACC86200.025.1902562
AAAGTCG136600.024.80746794
CGTGCGG85950.024.607339
GACCCGT96450.022.5619495
TCGACCC104150.021.2549213
ACGCGCG69650.021.22254232-33
CGGGACG50000.020.21000190-91
ACCCGTG116400.018.856536
GGACGCG79200.018.75252730-31
GCGACAT61000.018.2606567
GCGCGAG85900.017.23515934-35
GCGCGGC83750.017.00417976-77
TGCGACA66550.016.4552976
Produced by FastQC (version 0.11.5)