FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49032422
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG361100.073.20077594
CAAGTCG221600.039.19494694
TAAGTCG147600.037.9566494
GAAGTCG299100.035.2303694
AAAGTCG119600.024.87541894
CGCGGAC41600.015.1394239
CCGCGGA43550.015.0011498
TATCCGC44150.014.2650065
ACAAGTC317050.013.08973494
GGCGCGT32500.013.0153851
ATATCCG46900.012.92750554
CTTGAAC603100.012.1727731
CGCGTGC47150.012.1611881
ATCCGCA65700.012.0898026
TTGAACC500300.011.3014182
CGAAGTC74400.011.18145294
GAACCCG114600.010.9092494
AGAAGTC516000.010.72984594
AACGCGC7051.1041266E-810.6666675
ATGCGCG11600.010.5344828
Produced by FastQC (version 0.11.5)