FastQCFastQC Report
Wed 13 Jul 2022
EGAF00006067919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006067919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343288801
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT17205250.5011887935138322Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC7415310.21600792039819555No Hit
AGTGATTAGCATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA6965410.20290233703254423No Hit
AGTGATTAGCAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT6868990.20009362321143706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)