FastQCFastQC Report
Thu 12 May 2022
EGAF00006070095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101524194
Sequences flagged as poor quality0
Sequence length8-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGAGGA4837058447.64439105027517No Hit
TANGAGGA4693560.4623095062443934No Hit
NACGAGGA3700530.36449735321218113No Hit
TACGAGGG3212090.3163866536088925No Hit
TACGAGGC3002270.2957196587051949No Hit
TAAGAGGA1641470.16168264285850917No Hit
TACGAGAA1220340.12020189000466235TruSeq Adapter, Index 20 (100% over 8bp)
TACGAGCA1027360.10119361302193643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)