FastQCFastQC Report
Sun 15 May 2022
EGAF00006070140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109829332
Sequences flagged as poor quality0
Sequence length8-151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTAGCA5372643448.91811050985906No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG21507971.9583083688426695No Hit
CGCGCGCCCTCCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGA18637651.6969647052027959No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA13531611.232057935124289No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC10337280.9412130449814627No Hit
GGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGG9785090.8909359477848776No Hit
GAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGACCGGGGTC7992120.7276853873608191No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC5471150.49815016629619496No Hit
TGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAG5347330.4868763109658174No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG4324650.39376093082310654No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGAC4100630.373363829618849No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA3519050.3204107623999753No Hit
NACTAGCA3482020.31703916764239265No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT3447450.31389155676554603No Hit
CTCGCGCGCCCTCCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGA3446470.3138023274146837No Hit
TGAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGACCGGGGT3282970.29891559387796335No Hit
ATCGCGCGCCCTCCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGA3082450.28065817608724053No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC3051880.2778747666424849No Hit
TCGCGCGCCCTCCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAG3029760.2758607327230216No Hit
AGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA3027360.275642211863767No Hit
GGTGAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGACCGGG2490430.22675454313060922No Hit
GGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGG2187350.1991590006210727No Hit
GACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCG2153760.19610061909508836No Hit
GTGAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGACCGGGG2103920.19156266925123427No Hit
TGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGG2012390.18322882998141152No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGCAATCTCGTA1958620.178333052230528TruSeq Adapter, Index 7 (97% over 35bp)
AATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAG1947800.1773478873567218No Hit
TTCGCGCGCCCTCCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGA1859880.1693427398793612No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG1775720.16167994174816613No Hit
CAACCCCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCC1762340.16046168795782167No Hit
AAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCC1755150.1598070358836381No Hit
CACTAGCG1604040.1460484162828196No Hit
CAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACG1585090.1443230119982884No Hit
AACCGAAGAAGCGGGGCGCGCCGACCGGGGTCGCGCGCCCTCCCCCCCAC1487760.13546108065193369No Hit
AGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGG1409010.12829086495764172No Hit
GTCGCGCGCCCTCCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGA1311810.11944077015782997No Hit
ATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGG1309710.11924956440598218No Hit
TACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGA1304360.11876244499056045No Hit
AGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGA1282680.11678847322862712No Hit
CCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGC1256100.1143683547123823No Hit
AACCCCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCG1253150.11409975615621518No Hit
CACTAGCC1203950.10962007854149565No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1159390.10556287458800169No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1150380.10474251086221667No Hit
CCAACCCCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGC1128120.10271572989263014No Hit
CGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGC1118710.10185894602363602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)