FastQCFastQC Report
Thu 12 May 2022
EGAF00006070141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109829332
Sequences flagged as poor quality0
Sequence length8-151
%GC61

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGTC5357753648.78253834777034No Hit
TTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG19633751.7876599668292619No Hit
TCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGT15344011.3970776040047297No Hit
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG9961260.9069762893577464No Hit
TCCGTCCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGT7282730.663095173883057No Hit
CCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGG7000960.6374399145029854No Hit
TTCCGCTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG5999870.5462903115899859No Hit
CGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGG5386430.49043637996450706No Hit
GCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGAC4795590.43664018643034264No Hit
GTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGC4121010.3752194359153528No Hit
CTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGA4088050.37221841611492273No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3982890.36264356046524987No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGC3622240.32980624884434334No Hit
TCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAG3256540.2965091329154219No Hit
CCCGGTCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGG3025090.2754355275510553No Hit
NTCTTGTC3019300.27490834597810354No Hit
TCCGCTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGT2831040.2577672055767397No Hit
CCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACC2753030.25066436714738466No Hit
CCCGGGCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGG2707410.24651064981438656No Hit
GTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGG2322500.21146445650784804No Hit
GGCTTGTC2260980.20586303848228815No Hit
TTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG2169390.197523736190984No Hit
GGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCC2098660.19108374436803457No Hit
TCCGTACGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGT2085370.18987368510991218No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCTTGTCGTGTAGATC1979310.1802168841380188Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGG1976930.18000018428592465No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG1951360.1776720266312828No Hit
TCCGGTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGT1924240.17520274092170568No Hit
TCCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG1845810.16806166134198103No Hit
TTCCATTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG1745800.15895571503612532No Hit
CCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGT1716710.1563070601212434No Hit
TTCCGATCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG1702710.15503235510892482No Hit
TCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGG1647750.1500282274319942No Hit
GGCGTCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCC1582490.14408628106742924No Hit
TCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGG1552210.1413292762265002No Hit
TCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGG1548440.14098601637675445No Hit
TCCGATCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGT1491420.13579432496229696No Hit
CCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGG1483850.1351050737520647No Hit
CTCCCAGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGA1438070.130936788361783No Hit
GCCTTGTC1389960.12655635563730824No Hit
TTCCTTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG1367270.12449042301377194No Hit
CCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGG1297240.11811416644143843No Hit
CGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCG1290730.1175214286107103No Hit
GTCCTCCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGC1269950.11562940217099746No Hit
TTCTGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG1253560.11413708680300451No Hit
GTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG1138550.10366538512680747No Hit
TTTCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG1132730.10313547204311505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)