FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10601447
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC110010.10376885344047845TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19900.027.36051842
CGTATGC20500.027.10860344
TCTCGTA20650.026.58469841
TCGTATG21150.025.8498443
ATCTCGT22250.024.6729940
CATCTCG22550.024.24451639
GATCGGA25250.021.4274731
GTATGCC27900.019.918645
ATCGGAA30500.017.7803632
TCGGAAG31000.017.6378443
CGTCTGA30600.017.57286516
ACACGTC31100.017.43478613
TATGCCG6350.016.65291245
CGGAAGA33400.016.6399254
ACGTCTG32950.016.31956315
CACGTCT33200.016.26421414
GCACACG35100.015.96063311
GATCATC36850.015.26379236
CACACGT38900.014.45933412
AGTCACC43750.012.95957228
Produced by FastQC (version 0.11.5)