FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11746558
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC4300.017.2669633
ACGTCCG4600.016.14085232
CTCGTAT5200.014.71087644
GTCCGCA5350.014.29903134
TCTCGTA6050.013.75971143
AACGGGA30750.012.877258
AAACGGG36400.012.6710797
TCCGCAC6050.012.64459735
CGTTGGA34300.012.536971
CGCACAT7500.012.29939537
TCGTATG6350.012.04690645
ACGGGAT23400.012.0182819
GAAACGG38700.011.9194386
CGGGATT22750.011.67005310
ACCGCGC9350.011.06920831
CCCGTAC1853.008811E-410.9527731
GGAAACG40050.010.8996385
CCCGAAA5850.010.3910921
ATCTCGT8050.010.34079642
ACACGTC9100.09.64344913
Produced by FastQC (version 0.11.5)