FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8803444
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC388040.4407820393927649TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT44850.040.93249542
CGATCAG45550.040.4562333
CGTATGC45550.040.451444
TCGTATG45600.040.3577143
TCTCGTA45750.040.12772441
ATCTCGT46450.039.52340
GTCACGA46900.039.096729
ACGATCA47750.038.732132
GATCGGA48800.038.499041
TCACGAT48250.038.04986230
AGTCACG48800.037.75783528
ATCGGAA50200.037.6974332
TCGGAAG50500.037.4734843
ACACGTC49900.037.4665313
CGTCTGA50250.037.11602416
CACGATC50750.036.3538531
ACGTCTG51700.036.29263315
CGGAAGA52700.035.7796134
GTATGCC51800.035.61411745
CACGTCT53550.035.0808414