FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6526157
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18550.013.7403361
AACGGGA14750.013.1142358
AAACGGG18850.012.8869147
ACGGGAT12400.012.5159539
CGGGATT11450.012.37489810
GAAACGG19550.012.3110056
CGCGCCC3950.011.95727333
CACCGCG4850.011.5933330
GGAAACG20350.011.4157795
CCGGATT1802.2937512E-411.2780192
ACCGCGC4800.011.24552931
GCCACCG13850.010.71792628
CCGCGCC5500.010.22320832
CCCGGAT2657.199482E-610.2140541
CGGCTAA6250.09.71613839
CCGGCTA6500.09.68845738
AACGGGT3951.22727215E-89.680298
CGCCCGG6450.09.41486235
GCGTGAG16350.09.21715522
ACAGGCG19900.08.92902218
Produced by FastQC (version 0.11.5)