FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11231073
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6050.024.8102841
ATCGGAA7900.019.3717442
ACACGTC8050.018.44621713
CGTCTGA8200.017.9255516
GTCACGT8600.017.44115829
AGTCACG8950.016.92669328
ACGTCTG9050.016.4076715
TCGGAAG9200.016.3068873
CGGAAGA9900.015.4568834
CACGTCT10400.014.85497314
GCACACG10500.014.28488511
TCACGTG10750.014.09245730
CACACGT11850.012.91064412
CGTTGGA26150.012.5133021
ACGGGAT15850.012.207719
AACGGGA21200.012.098578
AAACGGG27900.011.6659897
GAAACGG28350.011.6395356
CGGGATT15950.011.56672710
GGAAACG29100.011.2388135
Produced by FastQC (version 0.11.5)